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In contrast to the large amount of information available on the effect of hypoinsulinemia on P450 expression, few studies have investigated the P450 expression in animals with a type 2 diabetes-like syndrome, and these studies have reported, in part, contradictory results ( David Tate Newport Wedge xWEBFHBN
). Miu Miu Espadrille platform sneakers tOO8ps2
found that type 2 diabetes had no effect on the microsomal activity of CYP1, CYP2B, CYP2E, CYP3, and CYP4A in the obese-hyperglycemic ( ob / ob ) mouse. In contrast, Veja Mens Holiday Runner Trainers BMesh UK 7 TJDnMr
reported a decrease in CYP2E1 activity and an up-regulation in Cyp4a11 and Cyp4a14 mRNA in the same mouse model.

It seems reasonable to assume that these seemingly conflicting results are due to a varying expression of the enzymes at different stages of the development of insulin resistance and diabetes. Thus, we felt that a comprehensive comparison of the P450 and GST expression in mouse populations with different, well-defined metabolic abnormalities of glucose homeostasis was necessary. The objective of this study, therefore, was to compare hepatic P450 and GST expression in the three groups of NSZO mice that are normoglycemic/normolinsulinemic, hyperinsulinemic, or hyperglycemic/hypoinsulinemic. In addition, comparisons in expression were made between male and female NSZO mice in an attempt to find associations between gender-specific gene expression and the development of the type 2 diabetes-like syndrome.

The research was approved by the Animal Experimentation Ethics Committee at the Regierungspräsidium Cologne, Germany. SJL and NZO mice were obtained from Bomholtgard (Ry, Denmark). Housing and generation of F1 hybrids (SJL × NZO) and back-crosses (NZO × F1) were as described previously by this group ( Dorothy Perkins CLIO Espadrilles blue PIXjmH7T
). In brief, after weaning (3 weeks of age), mice were maintained on either a standard rodent food (1314) or a fat-enriched diet (C1057; Altromin, Lage, Germany). The high-fat diet contained 16% fat, 46.8% carbohydrates, 17.1% protein, and 15.4 kJ/g digestible energy. The standard diet contained 5% fat, 48% carbohydrates, 22.5% protein, and 12.5 kJ/g digestible energy. Mice were killed at the age of 22 weeks in isoflurane anesthesia by exsanguination. Blood was then collected, and livers were immediately excised and snap frozen in liquid nitrogen. Samples were stored at −70°C until required.

Blood glucose, serum cholesterol, and serum triglycerides were measured by AutoAnalyzer (Johnson Johnson, Neckargemünd, Germany). Plasma insulin was determined in duplicate by radioimmunoassay (Amersham Biosciences, Freiburg, Germany) with anti-rat insulin antiserum and 125 I-labeled rat insulin as tracer. Free and bound radioactivity were separated with an anti-IgG antibody and samples were measured for activity using gamma scintillation counting. Plasma free fatty acid (FFA) content was measured using a FFA determination kit (Roche Diagnostics, Mannheim, Germany) (Table 1 ).

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In This Section

The Author

Jean Louis Sébédio William W. Christie

UMR 1019, Unité de Nutrition Humaine, Plateforme d’exploration du métabolisme, INRA centre de Theix, 63122 St Genes Champanelle, France Brief Biography Jean Louis Sébédio James Hutton Institute (and Mylnefield Lipid Analysis), Invergowrie, Dundee (DD2 5DA), Scotland Brief Biography William W. Christie

1. Introduction

polyunsaturated fatty acids (PUFA) of C, C, and C chain lengths having up to six ethylenic bonds are part of the human diet. They are formed during heat treatments such as deodorization of vegetable or fish oils [1] or during frying operations [2]. This process tends to cause isomerization of double bonds without changing their positions to a significant extent. Some polyunsaturated fatty acids are also from natural origins such as those containing conjugated double bonds or conjugated linoleic acid (CLA). This short review will only deal with polyunsaturated fatty acids having methylene-interrupted double bonds as information on Valentino Black Fly Me To New York Sock Sneakers YL4XWY

A nutritional intervention carried out on human has shown that, like the monounsaturated fatty acids, some of the -PUFA may have an effect on lipoprotein metabolism [3]. It is therefore very important to detect and quantify them in food products. This brief review will describe the two main approaches, gas-liquid chromatography and high-performance liquid chromatography (together with chemical degradative techniques) used to analyse polyunsaturated fatty acids in food products.

2. Gas-Liquid Chromatography

Isomers of linoleic and α-linolenic acids

The three geometrical isomers of linoleic acid, namely 9,12-18:2, 9,12-18:2 and 9,12-18:2, which are very often present in refined, unhydrogenated liquid vegetable oils, are readily separated in the order stated on capillary columns coated with polar cyanosilicone. The four common geometric isomers of α-linolenic acid, i.e., 9,12,15-18:3, 9,12,15-18:3, 9,12,15-18:3 and 9,12,15-18:3, which are also very often present in refined liquid vegetable oils, give peaks that can be readily recognized in GC analyses on cyanosilicone capillary columns, and they are eluted from the column in order stated above ( Fig. 1 ) [4,5].

The 18:2 isomer group of partially hydrogenated vegetable oils may contain up to 15 other 18:2 isomers. The identification of these is difficult, because suitable commercial standards are not available, except for 9,12-18:2, 9,12-18:2 and 9,12-18:2. However, these are the isomers most often encountered and their elution patterns have been established for SP-2560 and CP-Sil 88 columns. In general, such isomers elute in the order < < followed by . We will not extend discussion on this subject because of the efforts made by the industry to reduce the amount of partially hydrogenated oil in human diet. However, pertinent information has been published [6].

Even greater care must be taken when analysing the 18:3 isomers present in some refined vegetable oils. 11-Eicosenoic acid (11-20:1) is a natural monounsaturated fatty acid present in appreciable amounts in some vegetable oils, such as peanut oil and canola oil. Animal fats, especially lard, also contain this fatty acid but at lower levels. The 11-20:1 elutes in the 18:3 region of the chromatogram and its relative retention time with respect to the 18:3 varies with the column temperature. Depending on the temperature of the column, it may elute before, with or after α-linolenic acid (9,12,15-18:3). Therefore, an understanding of these variations is critical for the correct identifications of all the peaks in the 18:3 region of the chromatogram and for achieving correct fatty acid compositional data (for details see [5,7]).

Isomers of eicosapentaenoic and docosahexaenoic acids

Studies carried out on the development of analytical methods for identifying and quantifying polyunsaturated fatty acids with 5 or 6 ethylenic bonds are rather scarce and literature on the subject is limited [8-11]. In one study [8], isomers of eicosapentaenoic (EPA, 20:5-3) and docosahexaenoic (DHA, 22:6-3) acids were prepared by isomerization with -toluenesulfinic acid and the isomers fractionated by silver ion HPLC in order to have pure fractions of mono- and di- isomers. BPX-70 columns seem to have a more suitable selectivity for the analysis of EPA and DHA isomers compared to SP-2560. Polyethylene glycol (PEG) phases may also be used to analyse isomers of long-chain PUFA in fish oil concentrates only if 22:0 and 22:1 are not present in significant amounts [9].

Two-dimensional fatty acid retention indices (for calculation see ref. 12) were found suitable and better than equivalent chain-length (ECL) values for identification of geometry in these polyunsaturated fatty acids [8].

Quantification of geometrical isomers of highly unsaturated fatty acids such as EPA and DHA is a complex issue considering the number of components and isomers that may result from a heat treatment [10]. A validated method was recently published by Fournier . [11] to quantify low amounts of geometrical isomers of EPA and DHA in refined fish oils. It consists of converting the fish oil in to methyl esters and then analysing these by GC on a 100 m CP-Sil 88 column using a temperature programming method and hydrogen as the carrier gas. The elution of the EPA and DHA geometrical isomers present in a fish oil deodorized at 220°C is presented in Figure 2 . The only drawback of such an analysis is the co-elution of a minor mono- isomer of DHA and all- DHA. However, for samples containing low levels of isomers this led to an underestimation of about 10%, which is within the magnitude of uncertainty of the method.

3. High-Performance Liquid Chromatography (HPLC)

For definitive identification of polyunsaturated fatty acids, it is necessary that they are each isolated in sufficient amount and purity for chemical characterization. Although silver ion TLC is useful for obtaining a total -18:3 fraction from isomerized oils [13], HPLC in the silver ion mode appears to be the most useful micropreparative method for isolation of individual isomers, as illustrated in Figure 3 for α-linolenic acid [14].

The all-isomer eluted first, followed by each of the di- isomers, then the mono- isomers and finally all--18:3. Similar results were obtained by Adlof, who was also able to resolve 15 of the 16 possible -isomers from arachidonate in the form of the methyl esters on a Chromspher Lipids column with hexane-acetonitrile (98.5:1.5, v/v) as mobile phase [15]. Later, Mjøs was able to separate methyl esters of isomerized EPA and DHA into groups according to the number of double bonds, although individual isomers were not resolved [8].

4. Position of a Double Bond in a Fatty Acid Chain

Determination of the position of a double bond on the carbon chain is a complex procedure ( Fig. 4 ), which involves a combination of partial hydrogenation of the purified fatty acid, isolation of the resulting monoenes and separation into and fractions by silver nitrate thin-layer chromatography or HPLC, and subsequent analysis of each fraction by GC coupled with Fourier-transform infrared spectroscopy (GC-FTIR) and by GC coupled with mass spectrometry (GC-MS) of the dimethyloxazoline (DMOX) derivatives.

Partial hydrogenation of the unknown polyunsaturated fatty acid is a critical step, which is usually carried out using hydrazine reduction as described by Ratnayake [16]. From a fatty acid having 3 double bonds, hydrazine reduction will give a mixture of the saturated fatty acid, 3 monoenes, 3 dienes and some unreacted fatty acid. As the hydrogenation takes place without modification of the position or the geometry of the original double bond, the double bonds in the three monoenes will be representative of the true positions. Consequently, structure elucidation is carried out on the monoenes. While the position of each ethylenic bond is given by GC-MS, the geometry of each double bond can be confirmed by GC-FTIR, although the elution characteristics on silver ion chromatography are usually a reliable guide.


Figure 1 . The 18:2 (left) and 18:3 (right) regions of a GC chromatogram of FAMEs from a refined canola oil sample analysed using a SP-2560 capillary column (100 m × 0.25-mm I.D. × 20 μm film thickness), operated isothermally at 180°C. Hydrogen carrier gas, flow rate 1 mL/min.


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Thiazolidinediones have been shown to decrease UAE in diabetics and the reduction in UAE has been associated with the thiazolidinediones-induced increase in serum adiponectin levels ( Miyazaki et al . 2007 ). These findings indicate that the beneficial effects of thiazolidinediones on diabetic nephropathy may be mediated by the thiazolidinediones-induced upregulation of circulating adiponectin which is PPARγ-dependent. Consistently, elevated serum adiponectin levels in PPARγ-agonist treated mice appear to increase recovery of injured podocytes ( Rutkowski et al . 2013 ).

Fenofibrate treatment decreases UAE, but the exact underlying mechanism remains unknown ( Keech et al . 2005 ). Moreover, fenofibrate has been reported to increase adiponectin expression in adipose tissue and serum adiponectin levels through PPARα activation and HDL elevation ( Christou Kiortsis 2013 ). Thus, the fenofibrate-induced elevation of serum adiponectin levels may contribute to the concomitant decrease in UAE, because of the renoprotective effects of adiponectin. Consistently, unpublished data from a previous trial performed by our study group showed that the percentage change in circulating HMW adiponectin over 3 months of fenofibrate treatment was negatively correlated with the percentage change in urinary albumin to creatinine ratio (ACR) ( r =−0.648, P =0.043) ( Christou et al . 2012 ).

Weight loss can induce a decrease in UAE in overweight or obese subjects ( Kiortsis Christou 2012 ). Long-term diets have been shown to increase serum adiponectin levels ( Christou Kiortsis 2013 ), indicating a possible role of adiponectin on mediating, at least in part, the weight loss-induced changes of UAE. There is a study mentioning that the change in serum adiponectin levels over 1 year of diet was not significantly correlated with the change in ACR ( Giuseppe Zanotti Chris Low ankle boots FfLQZAWOP
). Unpublished data from a previous trial performed by our study group showed that circulating HMW adiponectin at baseline was negatively correlated with the percentage change of logACR over 3 months of diet ( r =−0.769, P =0.003) ( Christou et al . 2012 ). Thus, high baseline serum adiponectin levels may predict a more favourable response of UAE during diet, possibly reflecting a higher grade of nephroprotection. Further studies are needed to elucidate the possible role of adiponectin in the weight-loss-induced decrease in UAE.

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Adiponectin is an adipokine that appears to have renoprotective effects through AMPK-activated pathways, resulting in the prevention of albuminuria. The relationship between circulating adiponectin and albuminuria has been reported to show a biphasic pattern. A negative relationship exists in the case of normoalbuminuria, possibly reflecting the increased renal clearance of adiponectin together with the renoprotective effects of adiponectin, and a positive association in more advanced albuminuria, indicating the counteracting upregulation of serum adiponectin levels to mitigate renal injury. Taking into account the fact that the currently believed route of renal biodegradation/excretion of adiponectin is not based on firm evidence, the confirmation of this route and the exploration of the exact related pathways are prerequisites for understanding the role of adiponectin with regard to renal physiology. In addition, the accepted renoprotective role of adiponectin has been based almost exclusively on data from nonhuman experiments, necessitating the performance of studies investigating the relevant issues in human experimental settings. Further clinical studies are needed to translate convincing experimental evidence for the nephroprotective role of adiponectin into clinical practice that could create new therapies for albuminuria, targeting specific pathways of adiponectin action.

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